Shodex HPLC Columns for Ion Exchange Chromatography (IEC)

Shodex Size Exclusion Columns for Polymers Chromatography with Organic Solvents

Shodex offers ion exchange chromatography (IEC) columns for the analysis of large biomolecules such as proteins, peptides, amino acids, DNA, and RNA.

In IEC, ionic attraction and repulsion forces separate components based on their ionic strengths. By altering the mobile phase’s electronic characteristics, components switch between cations and anions, leading to retention or elution from the column. Gradient methods involving mobile phase changes are common.

Shodex’s columns cater to various analysis needs, including non-suppressor and suppressor methods, transition metal ions, and rare earth metal ions and utilize strong and weak functional groups.

IEC QA-825

Polymer-based anion exchange columns

Contains strong quaternary ammonium groups
Ideal for analyzing relatively high molecular weight compounds such as proteins, peptides, DNA, and RNA
Usable across a broad pH range from pH 2 to 12

IEC DEAE-825
Asahipak ES-502N 7C

Polymer-based anion exchange columns

Features weak diethylaminoethyl groups
Asahipak ES-502N 7C uses polyvinyl alcohol as the base material, offering a distinct separation pattern
Low hydrophobic interaction with proteins allows analysis under mild conditions

IEC SP-825
IEC SP-FT 4A

Polymer-based cation exchange columns
strong Sulfopropyl groups
Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12

IEC CM-825
Asahipak ES-502C 7C

Polymer-based cation exchange columns
weak Carboxymethyl groups
Asahipak ES-502C 7C: polyvinyl alcohol is used as base material and this offers different separation pattern
Low hydrophobic interaction of proteins allows analysis under mild conditions