HPLC Columns – HILIC Columns

HILIC: Hydrophilic Interaction Chromatography

קולונות HILIC

Hydrophilic Interaction Chromatography (or Hydrophilic Interaction Liquid Chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography (RP HPLC). HILIC uses hydrophilic stationary phases with reversed-phase type eluents.

Functionalized polyvinyl alcohol gel gives the HILIC columns series a wide usable pH range (2 – 12), excellent chemical stability, and low shedding for sensitive detectors (MS, CAD, ELSD) .

Various Amino Functional groups complete the superior Performance of the Shodex HILIC columns for HILIC separations.

They Simplify HPLC methods by removing ion pair reagents and explore basic mobile phases. Tackle challenging separations with mulitmode capabilities (HILIC + Ion Exchange) and optimization for various Saccharides: Monosaccharides, oligosaccharides, Polysaccharides and other various compounds.

טכנולוגיה

Product: Asahipak NH2P HILICpak VG-50 & VT-50
Application: small polar molecules, saccharides

Fields: pharma, food, beverages, life science

HILIC: Hydrophilic Interaction Liquid Chromatography

Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period.

Suitable for polar analytes (like in IEX chromatography, IEC and IC)
Polar stationary phase (like in NP chromatography)
Polar mobile phase, e.g. water/ACN mixtures (like in RP chromatography)
Functional groups:
Amino (-NH2)
Diol
Zwitterionic
Pure silica

אפליקציות נבחרות
קולונות HILIC

HILIC Columns are used mainly for the separations of:

Various Saccharides (food and feed industry)

mono saccharides analysis

Oligosaccharides analysis

Polysaccharides analysis

Glyphosate analysis

קטלוג קולונות HILIC

HILIC - Chromatography

About HILIC

Professor Alpert introduced a new concept in 1990: hydrophilicity chromatography (HILIC). This chromatographic method is used to separate strong polar and hydrophilic compounds, such as nucleosides and nucleotides, amino acids, sugars, etc. Besides, it uses polar stationary phase, polar mobile phase, and solutions with lower polarity than stationary phase, such as acetonitrile/water, etc. Different from reverse phase chromatography, the greater the polarity of mobile phase, the stronger the elution ability in HILIC chromatography. Nevertheless, it is recommended that aqueous phase proportion should not exceed 40% or not less than 3%.