HILIC Columns – Hydrophilic Interaction Liquid Chromatography
HILIC:
Hydrophilic Interaction
Liquid Chromatography
Hydrophilic Interaction Chromatography (or Hydrophilic Interaction Liquid Chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography (RP HPLC). HILIC uses hydrophilic stationary phases with reversed-phase type eluents.
Functionalized polyvinyl alcohol gel gives the HILIC columns series a wide usable pH range (2 – 12), excellent chemical stability, and low shedding for sensitive detectors (MS, CAD, ELSD) .
Various Amino Functional groups complete the superior Performance of the Shodex HILIC columns for HILIC separations.
They Simplify HPLC methods by removing ion pair reagents and explore basic mobile phases. Tackle challenging separations with mulitmode capabilities (HILIC + Ion Exchange) and optimization for various Saccharides: Monosaccharides, oligosaccharides, Polysaccharides and other various compounds.
מידע נוסף
Main Applications
HILIC Columns are used mainly for the separations of:
Various Saccharides (food and feed industry)
mono saccharides analysis
Oligosaccharides analysis
Polysaccharides analysis
Glyphosate analysis
HILIC COLUMN Asahipak NH2P
Polymer-based HILIC column with amino (-NH2) groups
- Designed for polar substances
- Ideal for saccharide analysis using HILIC mode
- Polymer-based packing material ensures excellent chemical stability and minimal degradation over extended periods
- Easily regenerated by washing with an alkaline solution
- Suitable for ELSD, CAD, LC-MS/MS
- Meets USP L82 requirements
HILICpak VG-50
Polymer-based HILIC columns featuring tertiary amino (-NR2) groups
- Ideal for saccharide analysis in HILIC mode
- Enhanced recovery of reducing saccharides (notably galactose, mannose, arabinose, and xylose) compared to the Asahipak NH2P-50 series
- Excellent for melamine analysis
- Compatible with ELSD, CAD, LC-MS/MS
- Available in steel and PEEK housings to minimize interactions
HILICpak VT-50
Polymer-based HILIC columns featuring quaternary ammonium (-NR3+) groups
- Ideal for analyzing glyphosate and phosphorylated sugars
- Well-suited for anionic substances, particularly phosphate compounds, in HILIC mode
- Certain eluents can introduce ion exchange mode
- The polymer-based packing material offers exceptional chemical stability and minimal deterioration over long-term use
- Optimized for LC-MS/MS analysis
HILICpak VC-50
Polymer-based HILIC columns with carboxyl (-COO-) groups
- The modified carboxyl group is ideal for analyzing cationic substances, including amines
- Primarily utilizes RP or IEX modes over HILIC mode for separation
- Excellent for analyzing aminoglycoside antibiotics, histamine/histidine, monoamine neurotransmitters, and more
- Compatible with LC-MS/MS analysis
HILICpak VN-50
Polymer-based HILIC columns with diol (-OH) groups
- Ideal for oligosaccharides and oligonucleotides
- Suitable for separating oligomers, which is not achievable with SEC columns or conventional HILIC columns
- The modified diol groups on the packing material establish the HILIC mode
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HILIC Columns
Functional Groups
HILIC - Chromatography
Professor Alpert introduced a new concept in 1990: hydrophilicity chromatography (HILIC). This chromatographic method is used to separate strong polar and hydrophilic compounds, such as nucleosides and nucleotides, amino acids, sugars, etc. Besides, it uses polar stationary phase, polar mobile phase, and solutions with lower polarity than stationary phase, such as acetonitrile/water, etc. Different from reverse phase chromatography, the greater the polarity of mobile phase, the stronger the elution ability in HILIC chromatography. Nevertheless, it is recommended that aqueous phase proportion should not exceed 40% or not less than 3%.