Shodex Size Exclusion Columns for Peptides and Proteins Chromatography
Shodex Size Exclusion Columns
for Peptides and Proteins Chromatography -
Aqueous size-exclusion chromatography (SEC), also known as “Aqueous GFC,” is a well-established technique for protein characterization.
It has been used for quality control of biologics since the early days of recombinant protein development for pharmaceutical purposes.
SEC relies on highly porous particles packed into a chromatography column.
Based on the partitioning principle, larger molecules elute first, followed by progressively smaller sample components.
Two detection methods are commonly used: static light scattering for molecular mass determinations and the more classical approach of molecular size determination.
Shodex offers both silica-based and polymer-based aqueous SEC columns:
Silica-based columns, such as the PROTEIN KW-800 and PROTEIN LW, are suitable for analyzing proteins and enzymes.
Polymer-based columns like the OHpak SB-800 HQ cover a wide range of molecular weight sample analysis, including modified proteins, PEGs, polysaccharides, and surfactants.
מידע נוסף
PROTEIN KW series
PROTEIN LW series
- Silica-Based Columns for Aqueous SEC (GFC) Analysis
- Ideal for analyzing proteins and enzymes
- Meets USP L20, L33, and L59 standards
- LW-803: Specifically controlled pore size for high-performance analysis of antibody drugs and various proteins with molecular weights of several hundred thousand
- Available in smaller columns with reduced particle sizes
OHpak SB-800 HQ series
- Polymer-Based Columns for Aqueous SEC (GFC) Analysis
- Accommodates a wide range of molecular weight sample analyses
- Suitable for modified proteins, PEGs, polysaccharides (e.g., hyaluronic acid, alginates), and surfactants
- SB-807 HQ: Designed for analyzing water-soluble ultra-high molecular weight polymers, with large particle-size gel to prevent shear degradation
- Available in preparative dimensions as well
OHpak LB-800 series [for MALS]
- Similar to the OHpak SB-800 HQ series, but optimized for Light Scattering detectors
- Features higher crosslinking to ensure ultra-low bleeding, producing better chromatograms in particle-sensitive detectors
- Polymer-based columns designed for aqueous SEC (GFC) analysis
- Support a wide range of molecular weight sample analyses including modified proteins, PEGs, polysaccharides (such as hyaluronic acid and alginates), and surfactants
Asahipak GS series (multi mode)
- SEC is the primary separation mode
- The choice of eluent allows for multimode features, including reversed phase, HILIC, and ion exchange modes in addition to SEC
- Ideal for separating peptides or nucleic acids with similar molecular weights
- Suitable for desalting samples or substituting buffer in protein analysis
Ashipak GF series (multi solvent)
- Polymer-based SEC columns with high solvent durability
- Compatible with both aqueous and organic solvents
