Shodex Size Exclusion HPLC Columns for Peptides and Proteins Chromatography

Shodex offers a range of Aqueous Size Exclusion (SEC) HPLC columns
designed for analyzing proteins, enzymes, and other large biomolecules in aqueous solvents.

These columns come in both silica-based and polymer-based variants.

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Silica-based columns, such as the PROTEIN KW-800 and PROTEIN LW, are suitable for analyzing proteins and enzymes.

Polymer-based columns like the OHpak SB-800 HQ cover a wide range of molecular weight sample analysis, including modified proteins, PEGs, polysaccharides, and surfactants.

The polymer-based OHpak SB-800 HQ and SB-2000 columns support a wide range of molecular weight sample analyses, including modified proteins, PEGs, polysaccharides (like hyaluronic acid and alginates), and surfactants.

Silica gel columns generally provide better resolution due to their sharper pore size distribution and larger pore volume compared to polymer-based columns.

Shodex’s expertise in SEC ensures reliable and efficient separations for researchers

PROTEIN KW series
PROTEIN LW series

Silica-Based Columns for Aqueous SEC (GFC) Analysis
Ideal for analyzing proteins and enzymes
Meets USP L20, L33, and L59 standards
LW-803: Specifically controlled pore size for high-performance analysis of antibody drugs and various proteins with molecular weights of several hundred thousand
Available in smaller columns with reduced particle sizes

OHpak SB-800 HQ series

Polymer-Based Columns for Aqueous SEC (GFC) Analysis
Accommodates a wide range of molecular weight sample analyses
Suitable for modified proteins, PEGs, polysaccharides (e.g., hyaluronic acid, alginates), and surfactants
SB-807 HQ: Designed for analyzing water-soluble ultra-high molecular weight polymers, with large particle-size gel to prevent shear degradation
Available in preparative dimensions as well

OHpak LB-800 series [for MALS]

Similar to the OHpak SB-800 HQ series, but optimized for Light Scattering detectors
Features higher crosslinking to ensure ultra-low bleeding, producing better chromatograms in particle-sensitive detectors
Polymer-based columns designed for aqueous SEC (GFC) analysis
Support a wide range of molecular weight sample analyses including modified proteins, PEGs, polysaccharides (such as hyaluronic acid and alginates), and surfactants

Asahipak GS series (multi mode)

SEC is the primary separation mode
The choice of eluent allows for multimode features, including reversed phase, HILIC, and ion exchange modes in addition to SEC
Ideal for separating peptides or nucleic acids with similar molecular weights
Suitable for desalting samples or substituting buffer in protein analysis