-Shodex HILIC HPLC Columns
Hydrophilic Interaction Liquid Chromatography

HILIC chromatography uses hydrophilic stationary phases with reversed-phase type eluents. 

Functionalized polyvinyl alcohol gel gives the HILIC columns series a wide usable pH range 2-12, excellent chemical stability, and low shedding for sensitive detectors (MS, CAD, ELSD) .

Various Amino Functional groups complete the superior Performance of the Shodex HILIC columns for HILIC separations. 

לקבלת ייעוץ ורכישה של קולונות
Ion Chromatography השאירו כאן את הפרטים:

Shodex HILIC columns simplify HPLC methods by removing ion pair reagents and using basic mobile phases. These columns handle challenging separations with multimode capabilities and are optimized for various saccharides and other compounds.

מידע נוסף

Main Applications

HILIC Columns are used mainly for the separations of:

Various Saccharides (food and feed industry)

mono saccharides analysis

Oligosaccharides analysis

Polysaccharides analysis

Glyphosate analysis


Polymer-based HILIC column with amino (-NH2) groups

  • Designed for polar substances
  • Ideal for saccharide analysis using HILIC mode
  • Polymer-based packing material ensures excellent chemical stability and minimal degradation over extended periods
  • Easily regenerated by washing with an alkaline solution
  • Suitable for ELSD, CAD, LC-MS/MS
  • Meets USP L82 requirements

HILICpak VG-50

Polymer-based HILIC columns featuring tertiary amino (-NR2) groups

  • Ideal for saccharide analysis in HILIC mode
  • Enhanced recovery of reducing saccharides (notably galactose, mannose, arabinose, and xylose) compared to the Asahipak NH2P-50 series
  • Excellent for melamine analysis
  • Compatible with ELSD, CAD, LC-MS/MS
  • Available in steel and PEEK housings to minimize interactions

HILICpak VT-50

Polymer-based HILIC columns featuring quaternary ammonium (-NR3+) groups

  • Ideal for analyzing glyphosate and phosphorylated sugars
  • Well-suited for anionic substances, particularly phosphate compounds, in HILIC mode
  • Certain eluents can introduce ion exchange mode
  • The polymer-based packing material offers exceptional chemical stability and minimal deterioration over long-term use
  • Optimized for LC-MS/MS analysis

HILICpak VC-50

Polymer-based HILIC columns with carboxyl (-COO-) groups​

  • The modified carboxyl group is ideal for analyzing cationic substances, including amines
  • Primarily utilizes RP or IEX modes over HILIC mode for separation
  • Excellent for analyzing aminoglycoside antibiotics, histamine/histidine, monoamine neurotransmitters, and more
  • Compatible with LC-MS/MS analysis

HILICpak VN-50

Polymer-based HILIC columns with diol (-OH) groups

  • Ideal for oligosaccharides and oligonucleotides
  • Suitable for separating oligomers, which is not achievable with SEC columns or conventional HILIC columns
  • The modified diol groups on the packing material establish the HILIC mode

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HILIC - Chromatography

Professor Alpert introduced a new concept in 1990: hydrophilicity chromatography (HILIC). This chromatographic method is used to separate strong polar and hydrophilic compounds, such as nucleosides and nucleotides, amino acids, sugars, etc. Besides, it uses polar stationary phase, polar mobile phase, and solutions with lower polarity than stationary phase, such as acetonitrile/water, etc. Different from reverse phase chromatography, the greater the polarity of mobile phase, the stronger the elution ability in HILIC chromatography. Nevertheless, it is recommended that aqueous phase proportion should not exceed 40% or not less than 3%.