LUMERA Multi-Channel Protein Purification System
What is LUMERA?
LUMERA is an automated protein purification system built for biotechnology, pharmaceutical, academic and government research labs that need to scale throughput without scaling headcount. Unlike traditional single-channel FPLC systems, LUMERA processes up to 8 protein samples simultaneously using positive-pressure liquid delivery for consistent, synchronized results.
How do real-time UV detection and flow-through re-application improve yield?
Integrated real-time UV detection at 280 nm provides live visibility into column loading, breakthrough and elution, eliminating manual sampling and guesswork. LUMERA’s automated flow-through re-application — a capability unique to the platform — re-loads flow-through fractions to recover unbound target protein and maximize resin utilization, significantly improving overall yield compared with a gravity-column or standard FPLC workflow.
What can LUMERA purify?
LUMERA covers the major purification workflows and column formats:
- Antibody purification — Protein A, Protein G and Protein L affinity purification of monoclonal and polyclonal antibodies and fragments
- IMAC / His-tag — parallel purification of His-tagged recombinant proteins using Ni-NTA, Co-NTA or Cu-NTA resins
- Ion exchange (CEX / AEX) and hydrophobic interaction chromatography (HIC)
- Oligonucleotides and small biomolecules — using standard SPE cartridge formats
- Compatible with pre-packed columns (including GE HiTrap), self-poured, gravity-flow columns and SPE cartridges; supports 9 buffer solutions
LUMERA at a glance
| Specification | LUMERA |
|---|---|
| Samples in parallel | Up to 8 |
| Real-time UV detection | 280 nm (live trace) |
| Buffer solutions | 9 |
| Column compatibility | Pre-packed (incl. GE HiTrap), self-poured, gravity-flow, SPE cartridges |
| Throughput | Up to 40X faster than manual purification |
| Operation | Standalone touchscreen with UV trace — no external PC |
| Lab environment | Compact bench-top; cold-room / cabinet compatible |
Key features
- Purify up to 8 clarified extracts simultaneously
- Real-time UV detection at 280 nm — no manual sampling
- Automated flow-through re-application maximizes protein yield
- Automated online cleaning, re-equilibration and optional strip / re-charge
- Integrated touchscreen with UV trace visualization — standalone, no external PC
- Compact bench-top footprint, cold-room compatible
Frequently asked questions
What proteins can LUMERA purify?
Antibodies, enzymes, His-tagged recombinant proteins, oligonucleotides and other biomolecules using affinity, IMAC, IEX, HIC and SPE chromatography formats.
How does LUMERA compare to a traditional FPLC system?
Unlike single-channel FPLC systems that process one sample at a time, LUMERA purifies up to 8 clarified extracts simultaneously, delivering far higher throughput for the same hands-on time.
What columns does LUMERA support?
Pre-packed columns (including GE HiTrap), self-poured columns, gravity-flow columns and standard SPE cartridges.
What is flow-through re-application?
A LUMERA-exclusive feature that automatically re-loads flow-through fractions onto the column to recover unbound target protein, maximizing resin utilization and overall protein yield.
Does LUMERA require an external computer?
No. LUMERA features an integrated touchscreen with UV trace visualization and operates as a standalone instrument — no external PC or software license required.
Written by Dani Shahar and the BioAnalytics website team
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