Total Protein – Dumas Method

Exploring the Dumas Method: Total Protein Analysis in Food and Feed

The Dumas method is a well-established technique for determining both total nitrogen and protein content in food and feed.

  1. Dumas Method Overview:

    • The Dumas method, also known as the combustion method, is used to determine the nitrogen content in organic samples.
    • It was developed by the French chemist Jean-Baptiste Dumas in 1831.
    • The method involves combusting the sample at high temperatures in the presence of oxygen (O2).
    • The nitrogen in the sample is converted into nitrogen oxides (NOx) during combustion.
  2. Applications:

    • The Dumas method is globally recognized and validated by international organizations such as AOAC, AACC, ISO, DIN, ASBC, AOCS, and OIV.
    • It can analyze both organic and inorganic nitrogen components.
    • In addition to protein determination, it can also be used to determine nitrite (NO2) and nitrate (NO3) levels.
  3. Advantages:

    • Short analysis time: The Dumas method provides rapid results.
    • Simple operation: Modern automated combustion devices make it easy to integrate into laboratory workflows.
    • High safety: Automated systems ensure safety during the analysis.
  4. Applications in Food and Feed Industry:

    • The Dumas method is particularly useful for quality control and nutrition labeling in the food and animal feed industry.
    • It allows detailed analytics, which is essential for meeting industry demands.
    • Whether analyzing fertilizers in agriculture, whey products in the food industry, or sustainable trends like insect protein, the Dumas method delivers reliable results.

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The PrimacsSN-100 presents an ideal solution for laboratories requiring swift and precise analysis of proteins across a diverse range of food and feed samples. Utilizing automation, this system significantly reduces analysis durations and amplifies sample throughput.

Ensuring utmost accuracy, our analyzers undergo rigorous testing and calibration to align with each customer's standards, guaranteeing optimal and precise results.

Key attributes of the PrimacsSN-100 include a 100-position random access autosampler with the option to prioritize samples, reusable ceramic crucibles, and capacity for sample weights up to 3g of solid material and 1g of liquid material. Its vertical sample introduction system facilitates seamless insertion of samples into the high-temperature combustion furnace, preserving sample ashes within the crucible to prevent buildup in the combustion zone and minimize maintenance time.

The determination of Total Nitrogen (TN) and protein content relies on the Dumas method and Thermal Conductivity detection (TCD), offering a rapid, safe, and eco-friendly alternative to traditional Kjeldahl methods. Pre-loaded methods cater to various sample types, including cereals, dairy, starch, grains, meats, animal feed, malt, and more.

A unique temperature ramping program is integral to the PrimacsSN-100, particularly beneficial for samples prone to erratic combustion behaviors like meat and vegetable oil. This patented technique allows for customized combustion temperature profiles tailored to specific sample matrices, ensuring optimal combustion patterns for accurate analysis.

Overall, the PrimacsSN-100 delivers fast, precise, and reliable TN/protein analysis across a spectrum of food samples, facilitating uninterrupted operation for enhanced efficiency and high-quality results.

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